Composite

Part:BBa_K4695223

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-03)


dcx4-docxylY device

This device is a fully functional gene capable of expressing Dcx4-DocXylY fusion protein (BBa_K4695123).

Design Notes

This device uses:

  • AB_T7_lacO promoter (BBa_J435350)
  • BC_BCD23 RBS (BBa_J435385)
  • EF_T7term terminator (BBa_J435361)

All non-coding parts and linkers were chosen from the E. coli Protein Expression Toolkit collection.
This device is compatible with AF vectors.
FLANKED 5' fusion site: GGAG, 3' fusion site: CGCT.

Source

Dcx4:
Organism: Pantoea dispersa BJQ0007
Article: https://doi.org/10.1016/j.jfca.2021.104201

DocXylY:
Organism: Acetovibrio thermocellus
Structure: https://www.rcsb.org/structure/1OHZ

Registry of standard biological parts


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BamHI site found at 1253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 825
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None